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URL: http://www.addgene.org/62988
Proper Citation: RRID:Addgene_62988
Insert Name: hSpCas9-2A-Puro V2.0
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24157548
Vector Backbone Description: Vector Backbone:PX459; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: NOTE: This is an improved version of Addgene Plasmid #48139. It has improved puromycin selection. The EcoRI site is not unique and flanks the puromycin cassette. For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/ The guide sequence is cloned into this plasmid using BbsI sites. The hU6-F primer (5'-GAGGGCCTATTTCCCATGATT-3') can be used to confirm the gRNA sequence after cloning into the plasmid. Please see the cloning protocol provided by the Zhang lab for more details.
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No rating or validation information has been found for pSpCas9(BB)-2A-Puro (PX459) V2.0.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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