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Species: Other
Genetic Insert: H2B-sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113841 Copy
Species: Other
Genetic Insert: H2B-sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113840 Copy
Species: Other
Genetic Insert: sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113846 Copy
Species: Other
Genetic Insert: pac-T2A-iCre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function. We have also deposited a version of this plasmid without the extra Valine, plasmid #125821.
Proper citation: RRID:Addgene_113849 Copy
Species: Other
Genetic Insert: H2B-sfGFP-P2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please read a Figshare page (https://doi.org/10.6084/m9.figshare.13237328.v16) before ordering this plasmid. For unknown reasons, the P2A in this construct doesn't skip very well. The T2A versions do skip well (e.g., https://www.addgene.org/113839/).
Proper citation: RRID:Addgene_113847 Copy
Species: Homo sapiens
Genetic Insert: MSX2
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pMSX2-donor
Proper citation: RRID:Addgene_113793 Copy
Species: Homo sapiens
Genetic Insert: TCF15
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTCF15-donor
Proper citation: RRID:Addgene_113798 Copy
Species: Homo sapiens
Genetic Insert: TCF15 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: K562.
Use with plasmid pTCF15.1.0-gDNA
Proper citation: RRID:Addgene_113830 Copy
Species: Homo sapiens
Genetic Insert: HOXC13
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pHOXC13-donor
Proper citation: RRID:Addgene_113795 Copy
Species: Homo sapiens
Genetic Insert: DEAF1
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 62987); Vector Backbone:pX462; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. This is a nickase guide and must be used with the additional nickase plasmid pDEAF1.2.1-gDNA and donor plasmid pDEAF1-donor.
Proper citation: RRID:Addgene_113799 Copy
Species: Other
Genetic Insert: H2B-sfGFP-T2A-tdTomato
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113839 Copy
Species: Other
Genetic Insert: mTagBFP2-T2A-iCre
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that iCre in this plasmid has an extra Valine upstream of the SV40 nuclear localization signal that does not affect the function. We have also deposited a version of this plasmid without the extra Valine, plasmid #125822.
Proper citation: RRID:Addgene_113837 Copy
Species: Homo sapiens
Genetic Insert: HOXB5
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pHOXB5-donor
Proper citation: RRID:Addgene_113783 Copy
Species: Homo sapiens
Genetic Insert: GLIS3
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pGLIS3-donor
Proper citation: RRID:Addgene_113780 Copy
Species: Homo sapiens
Genetic Insert: TOX2
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTOX2-donor
Proper citation: RRID:Addgene_113787 Copy
Species: Homo sapiens
Genetic Insert: FOXF2
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pFOXF2-donor
Proper citation: RRID:Addgene_113784 Copy
Species: Homo sapiens
Genetic Insert: OVOL1 homology arms flanking EGFP-IRES-Neo cassette
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Cloning source: MCF-7.
Use with plasmid pOVOL1.1.0-gDNA
Proper citation: RRID:Addgene_113826 Copy
Species: Homo sapiens
Genetic Insert: ZNF215
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pZNF215-donor
Proper citation: RRID:Addgene_113771 Copy
Species: Homo sapiens
Genetic Insert: TCFL5
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pTCFL5-donor
Proper citation: RRID:Addgene_113775 Copy
Species: Homo sapiens
Genetic Insert: REST
Vector Backbone Description: Backbone Marker:Zhang lab (Addgene plasmid ID: 42230); Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA sequence listed includes 20 nucleotide target sequence, followed by the uncloned PAM sequence. The PAM sequence is not present in the plasmid. Use with plasmid pREST-donor
Proper citation: RRID:Addgene_113774 Copy
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