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URL: http://www.addgene.org/30125
Proper Citation: RRID:Addgene_30125
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:6136; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This is an empty LIC vector derived from pcDNA3. It adds an mCherry gene to the C-terminus of your protein of interest. mCherry has a excitation max of 587 nm and an emission max of 610 nm. To clone into this vector, add the following tags to your PCR primers: LIC vKoz Forward tag 5’-TACTTCCAATCCAATGCCACC(ATG) LIC vGFP Reverse tag 5’-CTCCCACTACCAATGCC Do NOT include a stop codon with your reverse primer. T4-treat PCR with dCTP. Linearize vector with SspI, then T4-treat with dGTP. Can verify the presence of insert by digesting with HindIII and XbaI. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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