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Plasmid Name
p206_LTJ_sgRNACD45.2_R3
RRID:Addgene_82674 RRID Copied  
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RRID:Addgene_82674
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Plasmid Information

URL: http://www.addgene.org/82674

Proper Citation: RRID:Addgene_82674

Insert Name: sgRNA targeting CD45.2 region 3

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:29445007

Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid# 48138); Backbone Size:9300; Vector Backbone:pX458; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin

Comments: Plasmid co-expresses Cas9 from S. pyogenes with 2A-EGFP, as part of the pX458 backbone. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544

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Data and Source Information

Source: Addgene