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URL: http://www.addgene.org/74843
Proper Citation: RRID:Addgene_74843
Bacterial Resistance: Chloramphenicol and Spectinomycin
Defining Citation: PMID:17690442
Vector Backbone Description: Backbone Marker:Hajdukiewicz P., Svab Z. and Maliga P.; Backbone Size:8500; Vector Backbone:pPZP221; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: Please note that pUC primers are not recommended for sequencing due to the inverted repeat of the Tnos sequence which forms a secondary structure and prevents sequencing reactions. If you need to sequence or PCR the Tnos region, digestion by AscI helps separates two Tnos sequences and prevents the secondary structure.
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Source: Addgene
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