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URL: http://www.addgene.org/60685
Proper Citation: RRID:Addgene_60685
Insert Name: M26I DJ-1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26234586
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This construct contains a Tobacco etch virus (TEV) protease recognition sequence (ENLYFQS) after the Thrombin recognition site, and just before DJ-1. (Ex. LVPRGSHMENLYFQSASKRAL......) with the TEV site italicized. Internal NdeI site of human DJ-1 eliminated by silent mutation (198A->T).
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Source: Addgene
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