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Plasmid Name
pHRIG-AktDN
RRID:Addgene_53597 RRID Copied  
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RRID:Addgene_53597
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Plasmid Information

URL: http://www.addgene.org/53597

Proper Citation: RRID:Addgene_53597

Insert Name: Akt1

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:23942361

Vector Backbone Description: Vector Backbone:pHR-IG (pHR’tripCMV-IRES-eGFP); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin

Comments: AktDN was purchased from Addgene (plasmid # 16243). The AktDN gene is an Akt gene where a point mutation in the 179th amino acid from the N terminal converts a lysine to a methionine. This mutated form of the Akt protein can still be phosphorylated at the S473 site but loses its ability to affect downstream factors.

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Data and Source Information

Source: Addgene