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URL: http://www.addgene.org/50853
Proper Citation: RRID:Addgene_50853
Insert Name: cofilin 1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18045906
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Human cofilin 1 cDNA was PCRed with a 5' primer that had an EcoRI, while the 3' primer had KpnI and EcoRV. EcoRI and KpnI were then used to ligate into pcDNA3.1- (leaving the EcoRV on the 3’ end of the insert). Forward primer sequence: CGGAATTCACCACCATGGCCTCCGGTGTGGCTGTCTC. Reverse primer sequence: GGGGTACCGATATCCTCACAAAGGCTTGCCCTCCAGG.
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Source: Addgene