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URL: http://www.addgene.org/48732
Proper Citation: RRID:Addgene_48732
Insert Name: mCherryS131C
Organism: Synthetic
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:21696135
Vector Backbone Description: Backbone Marker:QIAGEN; Backbone Size:3439; Vector Backbone:pQE-9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This plasmid must be grown under Ampicillin (100ug/ml) and Kanamycin (50ug/ml) selection in order to retain both the mCherryS131C plasmid and the pREP4 helper plasmid. Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results for recommended primers. The S131C mutation in this plasmid is S136C, when compared to the starting Met of wild-type mCherry.
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Source: Addgene
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