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URL: http://www.addgene.org/45874
Proper Citation: RRID:Addgene_45874
Insert Name: Semcap3 in situ probe
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Comments: For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 3184-3805 of AF127085.1, not bp# 3185-3804 as indicated on plasmid map. The plasmid functions as described in the associated publication.
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Source: Addgene
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