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URL: http://www.addgene.org/41739
Proper Citation: RRID:Addgene_41739
Insert Name: Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10882062
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Alternate plasmid name: pCS2 NLNR CC>SS-6MT All Notch1 constructs deposited here were cloned into the pCS2+MT vector (Rupp et al., 1994; Turner and Weintraub, 1994) and have the C-terminal 348 residues (aa 2185 to C terminus) of Notch replaced with a hexameric myc tag to facilitate biochemical analysis. PCR mutagenesis was used to create the C1675S and C1682S mutations in LNR (Addgene plasmid #41738).
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Source: Addgene
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