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URL: http://www.addgene.org/241315
Proper Citation: RRID:Addgene_241315
Insert Name: Mex3a
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pTRE2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Mex3a is fused to the HyperTRIBE domain and V5, followed by a t2a-mCherry. Mex3a underlying DNA sequence was altered to reduce GC content, but amino acid sequence is conserved to mm10 Mex3a. The "HyperTRIBE" mutation of the adar deaminase domain is E488Q in drosophila and is conserved at the E957Q amino acid in the mouse Adar gene (amino acid references transcript uc008pzx.2 which is 1178 amino acids in length). The Adar fusion insert is a C-terminal truncation of the total Adar gene from T788 to F1170 - which spans the deaminase domain
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Source: Addgene
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