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URL: http://www.addgene.org/208048
Proper Citation: RRID:Addgene_208048
Insert Name: BirAopt
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:37996419
Vector Backbone Description: Vector Backbone:Derived from Addgene ID# 52962 (Zhang Lab).; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Expresses optimized BirA only (with short C-terminal fusion derived from GSQ linker, RBXN polylinker, and P2A remnant). For performing BioE3 experiments; can be used for transient expression or packaged into lentiviral particles for stable cell line selection.
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No rating or validation information has been found for Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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