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URL: http://www.addgene.org/20595
Proper Citation: RRID:Addgene_20595
Insert Name: PRKAA1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17574021
Vector Backbone Description: Backbone Marker:William Hahn Lab (available at Addgene, #15300); Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: ORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags. This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations. Please see the following link for plasmids from this article that are not part of the kinase library http://www.addgene.org/pubmed/17574021 Addgene's sequencing results suggest that the best reference sequence for the PRKAA1 insert in this plasmid is AB022017.1.
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Source: Addgene
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