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URL: http://www.addgene.org/177335
Proper Citation: RRID:Addgene_177335
Insert Name: SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Organism: SARS-CoV-2 virus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34672947
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Silent mutation in the original sequence to delete a second NdeI site as NdeI was needed for cloning; codon coding for Q306 was mutated to codon coding for A306 to delete natural cleavage site of the 3CL-protease; codon coding for active site residue C145 was mutated to A145 for inactive protease production; Factor Xa cleavage site can cleave off all 3 downstream tags; three tandem FLAG epitope tags is followed by an enterokinase cleavage site.
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Source: Addgene
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