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Plasmid Name
INCTbiosyn-p35S-GFP(rc)phiC31
RRID:Addgene_127527 RRID Copied  
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RRID:Addgene_127527
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Plasmid Information

URL: http://www.addgene.org/127527

Proper Citation: RRID:Addgene_127527

Insert Name: EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.

Organism: Arabidopsis thaliana

Bacterial Resistance: Ampicillin

Defining Citation: PMID:32444777

Vector Backbone Description: Vector Backbone:pBluescript SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin

Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase phiC31 attachment sites sequences from Grandchamp, N. et al. PLoS One 9, (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).

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Data and Source Information

Source: Addgene