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URL: http://www.addgene.org/8707
Proper Citation: RRID:Addgene_8707
Insert Name: Stat3
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7774815
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pRc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Diagnostic Restriction Digest with BamHI (3.2kb, 2.8kb, and 2.0kb). Due to a stop codon error made when tagging the Stat3 clone with FLAG (DYKDDDDK) additional residues precede (SR) and follow the tag (GPYSIVSPKC STOP). The ApaI cloning site is GGG CCC, which encodes the G and P residues coming after the FLAG tag. Therefore, if the insert is removed with a NotI and ApaI digestion, it will not have a stop codon included. * E16K mutation found during QC
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Source: Addgene
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