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Plasmid Name
p242_LTJ_1kbCD45.1Template
RRID:Addgene_82661 RRID Copied  
PDF Report How to cite
RRID:Addgene_82661
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Plasmid Information

URL: http://www.addgene.org/82661

Proper Citation: RRID:Addgene_82661

Insert Name: 1kb genomic DNA sequence from murine CD45.1

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:29445007

Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:HDR template for gene editing; Bacterial Resistance:Ampicillin

Comments: This HDR template can be used in conjunction with sgRNA CD45.2 (Addgene ID 82672). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544

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Data and Source Information

Source: Addgene