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URL: http://www.addgene.org/74630
Proper Citation: RRID:Addgene_74630
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27079678
Vector Backbone Description: Backbone Size:9626; Vector Backbone:All-in-One_Nickase-D10A; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Puromycin resistance gene is only for transient expression along with Cas9 nickase. Thus, drug selection period should be tested for each experiment. The presence of cellular puromycin resistance could be monitored by performing a control transfection with an empty backbone vector of either AIO-GFP or AIO-mCherry in which GFP/mCherry expression level represents puromycin resistance level.
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Source: Addgene
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