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Plasmid Name
pcDNA3-Flag-Tet3 CD Mut
RRID:Addgene_72222 RRID Copied  
PDF Report How to cite
RRID:Addgene_72222
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Plasmid Information

URL: http://www.addgene.org/72222

Proper Citation: RRID:Addgene_72222

Insert Name: Ten-Eleven Translocation 3

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:21778364

Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins Note that an additional mutation, D1507E, is present in the Tet3 insert in this plasmid. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead). For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862

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Data and Source Information

Source: Addgene