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URL: http://www.addgene.org/71311
Proper Citation: RRID:Addgene_71311
Insert Name: Cas9 nickase
Organism: S. pyogenes
Bacterial Resistance: Ampicillin and Kanamycin
Vector Backbone Description: Backbone Marker:Thermo-Fisher; Backbone Size:3900; Vector Backbone:PCR2-1 TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
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Source: Addgene
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