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URL: http://www.addgene.org/71151
Proper Citation: RRID:Addgene_71151
Insert Name: Ascl2
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26412560
Vector Backbone Description: Backbone Marker:Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.); Backbone Size:8370; Vector Backbone:pLV-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The cDNA sequence of murine Ascl2 was amplified and subcloned into the pCR2.1 vector. From there it was excised via flanking EcoRI sites and inserted into the pLV-tetO vector via the EcoRI. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
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Source: Addgene
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