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URL: http://www.addgene.org/66088
Proper Citation: RRID:Addgene_66088
Insert Name: sgRNA for APs1
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25249454
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.
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Source: Addgene
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