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URL: http://www.addgene.org/64518
Proper Citation: RRID:Addgene_64518
Insert Name: Type II hexokinase gene, partial cds and promoter region
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11557773
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5598; Vector Backbone:pGL2-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: For stability the plasmid is maintained in E. coli SURE. For reporter studies it is propagated in E. coli SCS110 or other methylation deficient strain. Note: Addgene's quality control sequencing has identified a few single nucleotide discrepancies with the depositor's reference sequence. However, the plasmid accurately reflects the construct as it was used in the associated publication, and should function as described.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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