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Plasmid Name
pGfa(B3)-nLac
RRID:Addgene_53132 RRID Copied  
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RRID:Addgene_53132
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Plasmid Information

URL: http://www.addgene.org/53132

Proper Citation: RRID:Addgene_53132

Insert Name: Gfa2

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:16496373

Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin

Comments: This plasmid is a variant of pGfa2-nLac (Addgene plasmid #53126) constructed by inserting 3 copies of the gfa2 B segment (Besnard et al. 1991) into the Sma I site just upstream of the basal promoter. It has about 75 times higher activity than pGfa2-nLac in transfected cells, but does not work in transgenic mice; possibly the high level of expression it produces is toxic.

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Data and Source Information

Source: Addgene