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URL: http://www.addgene.org/46144
Proper Citation: RRID:Addgene_46144
Insert Name: EGFP/mCherry
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19415631
Vector Backbone Description: Backbone Marker:stratagene; Vector Backbone:pBluescript II KS+; Vector Types:Cre/Lox, Tol2 zebrafish transgenic expression; Bacterial Resistance:Ampicillin
Comments: This plasmid can be used to make a transgene that expresses EGFP but can be switched to express mCherry if inverted. This reporter is controlled by a 2.3 kb regulatory sequence consisting of 0.2 kb Xenopus EF1alpha enhancer and 2.1 kb zebrafish beta-actin 2 sequence including the 1.5 kb enhancer/promoter sequence, the 1st exon (86 bp) and part of the 1st intron (490 bp). To generate a complete intron, the splice acceptor from the rabbit beta-globin gene was placed upstream of the EGFP reporter and a synthetic splice acceptor was placed upstream of the mCherry reporter. The synthetic splice acceptor contains the zebrafish consensus sequence along with intronic and exonic splice enhancers. There are some discrepancies between Addgene's and the depositor's sequence--these do not affect plasmid function.
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Source: Addgene
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