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URL: http://www.addgene.org/45865
Proper Citation: RRID:Addgene_45865
Insert Name: Btg1 in situ probe
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19793993
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Comments: The Bgt1 fragment was amplified by RT-PCR using the following primer pair: TGCCATAGTTTGGACAGTACC CAAAATAGATGGTGGTTTGTGG For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 1004-1638 when compared to BC006834.1, not bp# 1004-1638 as indicated on plasmid map as indicated on plasmid map. The plasmid functions as described in the associated publication.
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Source: Addgene
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