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URL: http://www.addgene.org/45624
Proper Citation: RRID:Addgene_45624
Insert Name: Cav1 in situ probe
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19793993
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Comments: The Cav1 fragment was amplified by RT-PCR using the following primer pair: ACCTCTCTGGACTGGCAGAA AGTGTCGGCAAGACTGAAGG For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the Spe1 restriction digest and T7 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 1472-2124 of AB029929.1, but found single nucleotide mismatches at bp# 1501 & 1574. The plasmid functions as described in the associated publication.
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Source: Addgene
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