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URL: http://www.addgene.org/45603
Proper Citation: RRID:Addgene_45603
Insert Name: s100a10 in situ probe
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15664173
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Comments: Fragment was amplified using the following primers: GCCCAGGTTTCGACAGACT CCACTAGTGATAGAAAGCTCTGGA For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 21-275 of BC025044, but found single nucleotide mismatches at bp#129 & 190. The plasmid functions as described in the associated publication.
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Source: Addgene