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Plasmid Name
pCRII-Topo S100a10 in situ probe
RRID:Addgene_45603 RRID Copied  
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RRID:Addgene_45603
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Plasmid Information

URL: http://www.addgene.org/45603

Proper Citation: RRID:Addgene_45603

Insert Name: s100a10 in situ probe

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:15664173

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin

Comments: Fragment was amplified using the following primers: GCCCAGGTTTCGACAGACT CCACTAGTGATAGAAAGCTCTGGA For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation. Please note that Addgene's sequencing results match bp# 21-275 of BC025044, but found single nucleotide mismatches at bp#129 & 190. The plasmid functions as described in the associated publication.

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Data and Source Information

Source: Addgene