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URL: http://www.addgene.org/45288
Proper Citation: RRID:Addgene_45288
Insert Name: TLOC1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23764425
Vector Backbone Description: Vector Backbone:pLEX_970; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The initial pDONR223 clone was obtained from the CCSB Human Orfeome Collection. Upon sequencing it was found that the construct contained a 660 bp long variant as compared to the expected 1200 bp long version, which initially had been described. To determine if whether this shorter version is expressed in cancer cells, and exclude the possibility of this being a cloning artifact, we cloned TLOC1 from T47D cells and found both forms to be expressed. We re-cloned both the full length and shorter splice variants by ligation of BP flanked PCR products into pDONR223 by a BP Gateway reaction. TLOC1 was transferred into DEST vectors from TLOC1 containing pDONR223 vectors through a Gateway LR reaction.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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