Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/42919
Proper Citation: RRID:Addgene_42919
Insert Name: Ets-1 PNT domain (46-138)
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19681083
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4970; Vector Backbone:pGEX-4T-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The gene fragment encoding residues 46-138 of Ets-1 (including the PNT domain) was amplified from an Ets-1 plasmid using primers to insert 5’ EcoR1 and 3’ Not1 restriction sites for incorporation into a pGEX-4T-2 vector. The primers also encoded an amino-terminal TEV protease cleavage site (ENLYFQ//C) and a carboxy-terminal hexahistidine tag. For this amplification the following PCR primers were used: 5’- GCC GGA ATT CGT GAA AAC CTG TAT TTT CAG TGC TCC CAA GCC TTG AAA GCT -3’ 5’- GCC CCC TTT TGC GGC CGC CTA GTG ATG GTG ATG GTG ATG ACC TTT CAC ATC CTC TTT CTG -3’ The PCR-amplified fragments were digested with Not1 and EcoR1 and ligated to Not1/EcoR1- digested and CIP-treated pGEX-4T-2. The protein coding sequence of the Ets-1 PNT domain is as follows: SQALK ATFSGFTKEQ QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM NGAALCALGK DCFLELAPDF VGDILWEHLE ILQKEDVK Alternate plasmid names: GST-TEV-PNT(46-138)-His6, GST-PNT, pGEX-PNT, GST-PNT-pGEX, GST-ENLYFQC-Ets-1(46-138)-His6, GST-TEV-Ets-1(46-138)-His6, GST-Ets-1(46-138), pGEX-Ets-1(46-138), GST-Ets-1(46-138)-pGEX
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for GST-ENLYFQC-PNT(46-138)-His6.
No alerts have been found for GST-ENLYFQC-PNT(46-138)-His6.
Source: Addgene
Welcome to the Neuroscience Information Framework Project, designed to serve the biomedical research community. NIF maintains the largest searchable collection of neuroscience data, the largest catalog of biomedical resources, and the largest ontology for neuroscience on the web. We welcome all feedback and suggestions and are actively looking for resource providers to make their resources accessible through NIF. Learn about the tools available to help you share your data and discover a dynamic inventory of Web-based neuroscience resources.
