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URL: http://www.addgene.org/35199
Proper Citation: RRID:Addgene_35199
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:13081; Vector Backbone:pBID-UAS-GGi; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven RNA inhibition (RNAi). 2 inverted gateway cloning cassettes are flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, hsp70 basal promoter. ftz intron facilitates RNA hairpin formation and expression. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please note that the inverted sequences present can cause rearrangements of this vector. Recommend miniprep only and confirm plasmid map subsequent to amplification or cloning. After gateway recombination, recommend amplification in Stbl2 cells [Invitrogen Cat. 10268-019] and combining minipreps for Drosophila injection. Addgene's sequencing results indicated a single nucleotide mismatch at bp# 5286 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect plasmid function. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
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Source: Addgene
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