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URL: http://www.addgene.org/33130
Proper Citation: RRID:Addgene_33130
Insert Name: GFP with TDH3 5' and 3' UTR
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12022234
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: T7 promoter driven GFP with the TDH3 5' and 3' flanking sequences, and the TDH3 polyadenylation signal all cloned into pRS314 using NotI and EcoRI.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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