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URL: http://www.addgene.org/32599
Proper Citation: RRID:Addgene_32599
Insert Name: H2B-EGFP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15619330
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that the plasmid contains a duplication of the H2B-EGFP region as compared to the original depositor sequence. The depositor has confirmed that the plasmid functions as described in the associated publication. The coding sequence for the human histone H2B gene (X57127) was amplified from genomic DNA by PCR using Pfx Polymerase (Invitrogen). The resulting product was cloned into pCR4 TOPO (Invitrogen) to generate pH2B. The H2B fragment was then cloned into plasmids pEGFP-N1, pDsRed2-N1 pDsRedExpress-N1 (BD Biosciences, Inc) in order to generate plasmids pH2B-EGFP, pH2B-DsRed2 and pH2B-DsRedExpress (oligonucleotide sequences are available upon request). The resulting fusions were then re-amplified by PCR and cloned into the XhoI site of pCAGGS [19] to generate pCX-H2B-EGFP, pCX-H2B-DsRed2 and pCX-H2B-DsRedExpress.
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Source: Addgene
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