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URL: http://www.addgene.org/32379
Proper Citation: RRID:Addgene_32379
Insert Name: int Ms6; gfpm2+; xylEm; ttsbiA, ttsbiB
Bacterial Resistance: Hygromycin
Defining Citation: PMID:20692326
Vector Backbone Description: Vector Backbone:pMl603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
Comments: This is an improved Ms6 integration vector for stable gene integration in mycobacteria with codon optimized gfp and xylE expression as reporter genes; terminators to protect expression cassette against transcriptional interference. Please note that the conserved N->S point mutation found in the Addgene QC sequence of the Ms6 integrase gene is of no consequence and the plasmid is still fully functional for integration.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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