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Plasmid Name
pRK5 FLAG 17xS/T->A, catalytic dead lipin 1
RRID:Addgene_32008 RRID Copied  
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RRID:Addgene_32008
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Plasmid Information

URL: http://www.addgene.org/32008

Proper Citation: RRID:Addgene_32008

Insert Name: Lipin1

Organism: Mus musculus

Bacterial Resistance: Ampicillin

Defining Citation: PMID:21816276

Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function.

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Data and Source Information

Source: Addgene