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URL: http://www.addgene.org/30462
Proper Citation: RRID:Addgene_30462
Insert Name: HNF1a promoter
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10585563
Vector Backbone Description: Backbone Size:3812; Vector Backbone:pCSGFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The GFP variant in the plasmid backbone, pCSGFP2, contains the F64L and the S65T mutations resulting in 35 times brighter green emission (as described in Ikawa M et al., Curr. Top. Dev. Biol. 44 (1999), pp. 1–20) This construct is derived from the (−6 kb/−58)-GFP construct [Addgene plasmid number 30460] by replacing the EcoRI–HindIII fragment (−494 to −58) with the corresponding EcoRI–HindIII fragment (−494 to −207) of the 3Δ207 clone (as described in Zapp D et al., Mol. Cell. Biol. 13 (1993), pp. 6416–6426).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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