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URL: http://www.addgene.org/29524
Proper Citation: RRID:Addgene_29524
Insert Name: proMMP-7
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19654318
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:3700; Vector Backbone:pET23b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: MMP7 sequence in the plasmid is suitable for expression in bacteria but NOT in mammalian cells, as the signal sequence is not included. The MMP7 amino acid sequence starts at amino acid 18 when compared to GenBank accession number NP_002414.1. Note that a superfluous methionine corresponding to the start signal for translation is present at the N-terminus of the expressed protein Please note that the His tag present in the vector is not in-frame with MMP-7
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Source: Addgene
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