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URL: http://www.addgene.org/26501
Proper Citation: RRID:Addgene_26501
Insert Name: spp-8
Organism: S. mediterranea (planarian)
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases. Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence; these do not affect plasmid function.
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No rating or validation information has been found for pJC136.4-spp-8.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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