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URL: http://www.addgene.org/26325
Proper Citation: RRID:Addgene_26325
Insert Name: mmu-mir-122
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20413612
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: pre-miRNA hairpins and the surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST.
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Source: Addgene
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