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URL: http://www.addgene.org/25070
Proper Citation: RRID:Addgene_25070
Insert Name: LRRK2
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:18397888
Vector Backbone Description: Backbone Marker:Cookson Lab; Backbone Size:4300; Vector Backbone:pCMV-Tag 3B; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: An additional Myc tag has been added to the original vector. The 2XMyc vector was adapted to the invitrogen gateway system by insertion of a gateway cloning cassette. LRRK2 was cloned by gateway recombination. The domain structure and amino acid limits is approximate. According to depositor's domain organization, this construct contains the correct residues.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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