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URL: http://www.addgene.org/250486
Proper Citation: RRID:Addgene_250486
Insert Name: D_LN5N
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:41224122
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pAGM1311; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: GRASP kit for MoClo assembly of sPPR proteins is a library of 42 plasmids which can be combined to assemble sPPR proteins with 9, 14, or 19 motifs to target any RNA sequence of the same length. See the original publication for more information, and find code for analysis and script for GRASP assembly at https://github.com/farleykvdg/GRASP.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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