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URL: http://www.addgene.org/24782
Proper Citation: RRID:Addgene_24782
Insert Name: Ptet-Gfp-ASV
Bacterial Resistance: Kanamycin
Defining Citation: PMID:15933004
Vector Backbone Description: Backbone Size:0; Vector Backbone:pFLAME4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: 1.Blokpoel, M. C. J., R. O'Toole, M. J. Smeulders, and H. D. Williams. 2003. Development and application of unstable GFP variants to kinetic studies of mycobacterial gene expression. J. Microbiol. Methods 54:203-211. [PubMed] 2. 2.Bateman, B. T., N. P. Donegan, T. M. Jarry, M. Palma, and A. L. Cheung. 2001. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69:7851-7857. [PubMed]
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org