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URL: http://www.addgene.org/247111
Proper Citation: RRID:Addgene_247111
Insert Name: GFP Nanobody
Organism: Camellid
Bacterial Resistance: Kanamycin
Defining Citation: PMID:41351542
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression, Affinity Reagent/ Antibody, Maintenance in bacteria; Bacterial Resistance:Kanamycin
Comments: The gpdA-driven reverse tet transactivator protein and tet-on-minimal promoter were obtained from Dr. Fabio Gsaller and the construction of these two features is described here: https://pubmed.ncbi.nlm.nih.gov/36350143/ .The 4X HA-GFPNb-rnf4 fusion gene was synthesized with A. fumigatus codon bias by Genscript. The GFP nanobody sequence-Rnf4 fusion was taken directly from: https://pmc.ncbi.nlm.nih.gov/articles/PMC7332993/ .
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Source: Addgene