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URL: http://www.addgene.org/24588
Proper Citation: RRID:Addgene_24588
Insert Name: Gateway(TM) cassette
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA. pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1 VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org