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URL: http://www.addgene.org/24492
Proper Citation: RRID:Addgene_24492
Insert Name: Human Sirtuin 3 catalytically inactive mutant
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20203611
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: SIRT3 contains a mutation at amino acid Q32H. This mutation is present in the first 100 amino acids of SIRT3 which serve as its mitochondrial import signal. A peptide corresponding to the first 100 aa of SIRT3 is normally cleaved once the protein has been imported into the mitochondria and the active form of SIRT3 in the mitochondrial matrix therefore lacks this part of the protein. Therefore the mutation should not modify SIRT3 activity.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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