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URL: http://www.addgene.org/240634
Proper Citation: RRID:Addgene_240634
Insert Name: 30x 147-bp Widom 601
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:40291647
Vector Backbone Description: Backbone Marker:Erik Jorgensen (Addgene plasmid #73738); Backbone Size:2438; Vector Backbone:pMLS280; Vector Types:DNA amplification; Bacterial Resistance:Kanamycin
Comments: Cloning carried out as in: Dyer PN, Edayathumangalam RS, White CL, Bao Y, Chakravarthy S, Muthurajan UM, Luger K. Reconstitution of nucleosome core particles from recombinant histones and DNA. Methods Enzymol. 2004;375:23-44. doi: 10.1016/s0076-6879(03)75002-2. PMID: 14870657. DNA purification as in: Paintsil EA, Morrison EA. Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles. Methods Mol Biol. 2023;2599:163-175. doi: 10.1007/978-1-0716-2847-8_12. PMID: 36427149. Nucleosome core particle reconstitutions largely as in: Hammonds EF, Morrison EA. Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. Methods Mol Biol. 2023;2599:177-190. doi: 10.1007/978-1-0716-2847-8_13. PMID: 36427150. Exception: Suggest reconstitution molar ratios of 1:1:2 DNA:H3/H4-tetramer:H2A/H2B-dimer. Please visit https://doi.org/10.1101/2025.04.09.647968 for bioRxiv preprint.
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Source: Addgene
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