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URL: http://www.addgene.org/240634
Proper Citation: RRID:Addgene_240634
Insert Name: 30x 147-bp Widom 601
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:40291647
Vector Backbone Description: Backbone Marker:Erik Jorgensen (Addgene plasmid #73738); Backbone Size:2438; Vector Backbone:pMLS280; Vector Types:DNA amplification; Bacterial Resistance:Kanamycin
Comments: Cloning carried out as in: Dyer PN, Edayathumangalam RS, White CL, Bao Y, Chakravarthy S, Muthurajan UM, Luger K. Reconstitution of nucleosome core particles from recombinant histones and DNA. Methods Enzymol. 2004;375:23-44. doi: 10.1016/s0076-6879(03)75002-2. PMID: 14870657. DNA purification as in: Paintsil EA, Morrison EA. Preparation of Recombinant Histones and Widom 601 DNA for Reconstitution of Nucleosome Core Particles. Methods Mol Biol. 2023;2599:163-175. doi: 10.1007/978-1-0716-2847-8_12. PMID: 36427149. Nucleosome core particle reconstitutions largely as in: Hammonds EF, Morrison EA. Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA. Methods Mol Biol. 2023;2599:177-190. doi: 10.1007/978-1-0716-2847-8_13. PMID: 36427150. Exception: Suggest reconstitution molar ratios of 1:1:2 DNA:H3/H4-tetramer:H2A/H2B-dimer. Please visit https://doi.org/10.1101/2025.04.09.647968 for bioRxiv preprint.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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