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URL: http://www.addgene.org/23969
Proper Citation: RRID:Addgene_23969
Insert Name: Ub-G76V-eGFP-V5HIS_15aa
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:19782090
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3970; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please note that this plasmid does not express a functional V5-His tag. This deletion construct was used to determine the minimum peptide length of a C-terminal tag to allow degradation of UbG76V in cells depleted of p97. The translated fusion protein ends with the following sequence: SCRPL ESRGP FEGKP* For more information, see the associated publication.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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