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URL: http://www.addgene.org/235571
Proper Citation: RRID:Addgene_235571
Insert Name: sgRNA backbone
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38724747
Vector Backbone Description: Vector Backbone:pPB-U6; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Drives expression of a sub-cloned single guide RNA (gRNA) with enhanced scaffold to target epigenetic editing to a desired locus. gRNA are cloned using the BlpI and BstXI sites. This is a piggyBAC plasmid that integrates into the genome and can be selected for via Puromycin resistance. An additional tagBFP insert is expressed constituitively and indirectly indicates presence of the gRNA.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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